Journal: Neoplasia (New York, N.Y.)

Article Title: Impaired Lymphocytes Development and Xenotransplantation of Gastrointestinal Tumor Cells in Prkdc -Null SCID Zebrafish Model 1

doi: 10.1016/j.neo.2016.06.007

Figure Lengend Snippet: IF and RT-PCR analyses for activated lymphocytes. (A) IF detected by CD2AP marker. The CD2AP-reactive lymphocytes are present in the peripheral blood from both SCID and control zebrafish, suggesting that the cells are either activated T lymphocytes or NK cells. DAPI staining was used to detect the blood cells. (B) Double IF detected by CD2AP (Cy2) and NKP46 (Cy3) markers. The NKP46 is a specific marker for NK cells, whereas the CD2AP marker is reactive to both T lymphocytes and NK cells. (C) RT-PCR analysis. Glyceraldehyde 3-phosphate dehydrogenase gene detection was used as control. Nonspecific cytotoxic cell receptor protein-1 and NK specific protein (NK cell triggering receptor) gene detections indicate NK cell. TCR V(D)JC (Vb12-Cb1) and immunoglobulin (IgVH1-Cm) gene transcript detections reveal V(D)J recombination and heavy-chain rearrangement. (D) Counted numbers of cells depending on CD2AP or NKP46 positivity. Green arrowhead, positive for CD2AP; red arrowhead, positive for NKP46; white arrowhead, positive for both CD2AP and NKP46; blue arrowhead, lymphocytes negative for both CD2AP and NKP46; yellow arrowhead; red blood cells.

Article Snippet: Immunofluorescence (IF) experiments were performed by using the cell smears with the primary antibodies rabbit anti–CD2-associated protein (CD2AP) (sc-9137, Santa Cruz Biotechnology Inc.) and mouse anti-NKP46 (sc-53,599, Santa Cruz Biotechnology Inc.).

Techniques: Reverse Transcription Polymerase Chain Reaction, Marker, Control, Staining

Journal: Cells

Article Title: A Mouse Model to Study the Pathogenesis of γ-herpesviral Infections in Germinal Center B Cells.

doi: 10.3390/cells12242780

Figure Lengend Snippet: Figure 5. Virally induced constitutively active CD30 signaling results in more Cre-reporter+ cells within B cells. CAR//Cγ1-cre mice (dots) and LC30//Cγ1-cre mice (squares) were i.p. infected with MHV-68 hNGFR and splenic cells were analyzed 14 days post-infection. (A) Breeding scheme of LC30//Cγ1cre mice. The excision of the loxP-flanked stop cassette was followed by hCD2 reporter expression (Cre-reporter) and ligand-independent constitutively active CD30 signaling. (B) The splenic

Article Snippet: The CD19-PeVio770 (REA749), CD38-PeVio770 and human CD2 (hCD2)-PeVio770 (LT2) antibodies were all purchased from Miltenyi Biotech (Bergisch Gladbach, Germany).

Techniques: Infection, Expressing

Journal: iScience

Article Title: Artificial antigen-presenting cell system reveals CD28’s role in modulating T cell functions during human immunodeficiency virus infection

doi: 10.1016/j.isci.2024.110947

Figure Lengend Snippet:

Article Snippet: CD3 Antibody, anti-human, Biotin functional grade , Miltenyi , Cat#130-093-377; Lot: 5210202091; RRID: AB_1036126.

Techniques: Functional Assay, Purification, Recombinant, Blocking Assay, Activation Assay, Software